Cyclin D1 anticorps (AA 1-295) (Biotin)

N° du produit ABIN7505972

Cet anticorps anti-Cyclin D1 est un anticorps Souris Monoclonal détectant Cyclin D1 dans WB, IP, ICC, IHC (p), IHC (fro) et ICFC. Adapté pour Humain, Souris, Rat et Non-Human Primate.

Aperçu rapide pour Cyclin D1 anticorps (AA 1-295) (Biotin) (ABIN7505972)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Souris, Rat, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: DCS-6

Détail du produit anti-Cyclin D1 anticorps (Biotin)

Épitope: AA 1-295

Fonction: Anti-Cyclin D1 Biotin

Specificité: The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Aucune reactivité croisée: Cat, Mouton

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: recombinant human cyclin D1 (amino acids 1-295)

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2-10 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1

Sujet: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling